The Non-accessible (break) colour field is the odd one out among the route settings. It does not colour the route line. It tells step-free routing which marks in your artwork are stairs or barriers.
How the break colour is used
Anywhere a path is drawn in the break colour, or a separate line in the break colour crosses or overlaps a path, is treated as steps. Normal directions are allowed to use those segments. Step-free directions avoid them and route around instead. If your map has no stairs, you can leave this field blank.
Set it up
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Choose the break colour
In the Wayfinding tab, set Non-accessible (break) colour to a colour you will not use for anything else on the map.
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Mark your steps in that colour
In your level artwork, either draw the stair section of a path in the break colour, or draw a short separate line in that colour crossing the path at the steps.
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Or use the draw tools
Instead of editing the artwork, use the Barrier line tool under Draw wayfinding on the map to draw across steps or blocked ways directly on the map.
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Enable step-free
The break colour only changes routing once step-free is on.
See Turn on the step-free (accessibility) option.
Because the break colour is matched against your artwork, keep it distinct from your route line colours so nothing is mistaken for stairs.